Abstract
Biocatalytic synthesis of drugs is imperative to avoid the hazardous environmental effects of the pharmaceutical production processes. In view of this, biocatalytic synthesis of aspirin is discussed in the present work. A crude lipase extracted from Geobacillus sp. was crosslinked using N,N-methylenebisacrylamide (N,N-MBAAm) and two different initiators: ammonium persulphate (APS) and γ-irradiation. The crude lipase was crosslinked as such bypassing the separation procedures using a biocompatible crosslinker. The crosslinked lipase was characterized by various techniques and evaluated for its activity profile as a function of time, temperature, pH and substrate concentration and results were compared to its free form. The activity profile of the crosslinked lipase was observed to be only marginally lower than the free lipase. The crosslinked lipases were used as catalysts in the green synthesis of acetylsalicylic acid, aspirin, directly from the acetic acid. The reusability studies of the crosslinked lipases were carried out up to 10 cycles.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
