Abstract
In this work, the chlorothalonil derivative (i.e. hapten) was synthesized. The chlorothalonil artificial antigens were prepared via the hapten conjugated with carrier proteins by activated-ester and mixed-anhydride method, respectively. The antiserum were obtained through immunizing the New Zealand white rabbits with the immune antigen, and the appropriate concentrations of coating antigen and antibody of ELISA were determined with chessboard format. Meanwhile, the influence factors of washing processes were also investigated, which evaluated by the value of OD 490 . The results showed that the artificial antigens of chlorothalonil were successfully synthesized, and the antiserum titer was about 1∶1.0×104. The detection limit (IC 20 ) of ELISA was 0.0123ng/mL. The recoveries of ELISA were from 84.0% to 89.8%, Coefficient of variation ranged from 3.0% to 7.6%. And all the Cross-reactivity (CR) with chlorothalonil analogous structure compounds were less than 0.01%.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
