Abstract
In recent years, immunoassay using small molecular compound as hapten has been applied in many fields, such as food and drug, environmental protection, etc., which has achieved ideal detection results. Only when the small molecule compound can only be coupled with the carrier to form an artificial antigen, can it indirectly induce B cells to proliferate and differentiate by T cell epitope, and then specific antibodies can be produced. The synthesis of efficient artificial antigen is the premise and key to immunoassay. This essay is a literature review at home and abroad about the design and synthesis methods of small molecular haptens, the selection of carriers, the coupling methods of haptens and carriers, the purification and identification methods, which are involved in the synthesis of artificial antigens.
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