Abstract
The main objectives of this research are to synthesize chitosan nanoparticles of chloroform fraction of K. rotunda, to characterize the products, and to conduct a biological test on these products as an antioxidant. Chloroform fraction of K. rotunda was loaded on chitosan nanoparticles and then was prepared by ionic gelation of chitosan with sodium tripolyphosphat (Na-TPP) in various compositions. Characterization of the products were investigated for particle size, zeta potential, and morphology by Scanning Electron Microscophy (SEM). The biological activity of the products as an antioxidant was tested by the DPPH method. Results of this study showed that the nanoparticle can be synthesized at the concentration ratio of 10: 1 for chitosan/Na-TPP. The size were in the range of 172 to 877 nm, with a zeta potential of + 28.06 to + 38.03 mV. The nanoparticle was cylinders in shape and smooth in surfaces. The antioxidant activity of chitosan nanoparticles of chloroform fraction of K. rotunda showed less activity compared with the previous fraction.
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