Abstract
The antimicrobial potential of the synthesized ternary nanochitosan (NCS)/sodium alginate (SA)/microcrystalline cellulose (MC) (extracted from banana fiber) film in 4:16:1 ratio against Bascillus subtilis and E. coli are reported here. Initially the present work focuses on the synthesis and characterization of ternary nanochitosan (NCS)/sodium alginate (SA)/microcrystalline cellulose (MC) (extracted from banana fiber) film. The prepared NCS/SA/MC film sample was then characterized using advanced analytical techniques such as FT-IR, XRD, TGA, DSC and SEM studies. The obtained results of FT-IR studies clearly indicate that the nanochitosan gets effectively binded with the sodium alginate and the microcrystalline cellulose in the film form. The change in crystallinity and the increased thermal behavior was elucidated from XRD and TGA analysis respectively. The scanning electron microscopic (SEM) studies of NCS/SA/MC film showed that the prepared film has the improved rough surface morphology and fractured structure. The bactericidal action of NCS/SA/MC film determined using Muller Hinton Agar medium on two microbial strains (Escherichia coli and Bacillus subtilis) reveals that the prepared NCS/SA/MC film has the greater potential to kill the microorganisms to a greater extent and hence this NCS/SA/MC film was found to be suggested as the promising material for biomedical applications.
Highlights
Pathogenic bacteria remain a major health concern especially since it is responsible for causing a large number of deaths and hospitalizations each year [1]
This study addresses the ways of obtaining and using the blended form of nanochitosan and sodium alginate with microcrystalline cellulose extracted from the banana fiber in the form of film in 4:16:1 ratio as antimicrobial agents against gram positive bacteria Bacillus subtilis and gram negative bacteria E. coli due to the problem of resistance of many infectious agents to the usual treatments
The FT-IR spectral details of pure chitosan (Figure 4a) showed that the hydroxyl (OH) peaks can be assigned at 3454.75 cm-1 and alkyl C–H stretching vibration were identified as doublets at 2923.08/2830.05 cm-1 respectively [30,31]
Summary
Pathogenic bacteria remain a major health concern especially since it is responsible for causing a large number of deaths and hospitalizations each year [1]. The risk of infection caused by microorganisms is of major concern in clinical, pharmaceutical areas (drugs, medical devices, odontology, hospital surfaces, etc.) and as well as in the food industries (food packaging, storage, fresh products, etc.). About two million peoples were affected by the bacterial infections in hospitals each year and this was reported according to the centers of disease control and prevention, Department of Health and Human Services, USA [5]. Gram-positive organism including Bacillus subtilis and gram negative bacteria including E. coli, were among the top 10 most frequently isolated organisms which produces too many infectitious diseases to the humans, in severe cases lead to death too
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call