Synthesis and Evaluation of Anticancer activity of some new 3(2h)-one pyridazinone derivatives by Molecular Docking Studies

Abstract

The present research aims to synthesize new series of 3(2h)-one pyridazinone derivatives with a potential anti-oxidant activity. All the compounds were synthesized by the esterification reaction of 2-(4- Fluoro Phenyl)--butyric acid with sulphuric acid and ethanol followed by hydrazine hydrate to give substituted hydrazides and then hydrazone with substituted ketons, which are cyclized with aldehyde substitutes to give the [5-(2,5-Dichloro-phenyl)-2-oxo-3,4-dihydro-2H-pyridin-1-yl]-acetic acid(4-4chloro-benzylidene)-hydrazide derivatives. Structure of all derivatives was confirmed by Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (NMR) spectroscopy. All these compounds were demonstrated for in-vitro anti oxidant activity by using DPPH radical scavenging activity and hydrogen peroxide scavenging activity. Molecular Docking was carried out by cyclin dependent kinase protein and DNA-hexamer ATGCAT. Molecular docking studies of derivatives were carried out by AutoDock Vina molecular docking software. All the results were calculated by PaDEL software. Results of antioxidant activity revealed that all compounds are potent antioxidants at a concentration of 50µg/ml.