Abstract
The simultaneous determination of amino acids in solution is important for food and nutrient industries. This study investigated the feasibility of potentiometric titration with synergy interval Partial Least Square (siPLS) for the simultaneous determination of glycine, glutamic acid and phenylalanine in aqueous solution. The methods used were compared with the traditional Partial Least Square (PLS) that based on full pH range. The performance was evaluated by the Root Mean Square Error of Cross-Validation (RMSECV), the Root Mean Square Error of Prediction (RMSEP) and the correlation coefficient (R). By optimizing the pH region with siPLS, a good linear model was produced for the calibration set with correlation coefficient R of 0.9947, 0.9956 and 0.9913 for glycine, glutamic acid and phenylalanine, respectively. A single set of synergy mixtures was tested independently and good results were obtained. The results show that the siPLS method can locate the informative region by using a graphically-oriented interface which is more easily to use and interpret. The study proves the feasibility of potential titration with chemometrics in the simultaneous determination of amino acid mixtures without preliminary separation.
Highlights
The simultaneous determination of different amino acids in solution is especially important for food and biopharmaceutical manufacturing as well as other related industries (Wakayama et al, 2010)
The results demonstrate that the synergy interval Partial Least Square (siPLS) algorithm can avoid the loose of relevant information region which will improve the performance of calibration model
The results show that the using of selected variables by iPLS and siPLS method presents better predictions with lower errors in relation to the full pH region Partial Least Square (PLS) model
Summary
The simultaneous determination of different amino acids in solution is especially important for food and biopharmaceutical manufacturing as well as other related industries (Wakayama et al, 2010). In addition to the HPLC method, gas chromatography, capillary electrophoresis and liquid chromatography combined with mass spectrometry are developed for the determination of amino acids (Mudiam et al, 2012; Mohabbat and Drew, 2008; Smith, 1997). There are some cases that the amino acids in mixtures are with the same type but in different concentrations, such as some amino acids nutrient solutions (Ohtani et al, 2006; Cerdán et al, 2013) and amino acid mixtures that extracted from protein hydrolysates (Liebster et al., 1961; Moore and Stein, 1949) in food industry For these samples, a simple and efficient way other than the expensive and time consuming HPLC methods, for the simultaneous determination of different amino acids will be beneficial
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