Synaptic placement and distribution of feed-back input on cortical neurons combined with intrinsic signal optical imaging and multiunit recording

Abstract

Event Abstract Back to Event Synaptic placement and distribution of feed-back input on cortical neurons combined with intrinsic signal optical imaging and multiunit recording Katalin Sári1*, Fuyuki Karube1, Zsanett E-Sólyom1 and Zoltán Kisvárday1 1 University of Debrecen, Laboratory for Cortical Systems Neuroscience, Department of Anatomy, Histology and Embryology, Hungary In the cat visual cortex area 17 (A17) projects to and receives input from numerous cortical areas of which area 18 (A18) provides the heaviest exchange of connections with A17 Indeed feed-back from A18 to A17 represents a substantial proportion of all input to A17 although the functional significance and termination pattern of synapses at the single cell level is not known. Here we used brain imaging, electrophysiological recording and morphological tools to reveal the contribution and significance of connections between A17 and A18. The border zone between A17 and A18 was visualised using activity maps obtained with intrinsic signal optical imaging based on the different spatial and temporal frequency preferences between A17 and A18 neurons. Next, multi unit activity was recorded at several locations in both areas in order to determine preferred orientation, direction and retinotopic position. Finally, extracellular microinjection of BDA was carried out at each electrode penetration. Anterogradely labelled axons running across the the border between A17/A18 and retrogradely labelled cell bodies were reconstructed in three-dimension using the Neurolucida reconstruction system. We expect to reveal corticocortical feed-back connections between the two early visual areas and determine the location of feed-back connections on the target cells. Combination of brain imaging and multiunit recordings will enable us to compare the functional characteristics of feed-back connections with the synaptic placement and distribution on visual cortical neurons. Supported by the European Consortium, FACETS (FP6-2004-IST-FETPI) and the Hungarian Academy of Sciences (TKI-242) to Z.K. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Abstracts Citation: Sári K, Karube F, E-Sólyom Z and Kisvárday Z (2010). Synaptic placement and distribution of feed-back input on cortical neurons combined with intrinsic signal optical imaging and multiunit recording. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00259 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 05 May 2010; Published Online: 05 May 2010. * Correspondence: Katalin Sári, University of Debrecen, Laboratory for Cortical Systems Neuroscience, Department of Anatomy, Histology and Embryology, Debrecen, Hungary, sari.katalin@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Katalin Sári Fuyuki Karube Zsanett E-Sólyom Zoltán Kisvárday Google Katalin Sári Fuyuki Karube Zsanett E-Sólyom Zoltán Kisvárday Google Scholar Katalin Sári Fuyuki Karube Zsanett E-Sólyom Zoltán Kisvárday PubMed Katalin Sári Fuyuki Karube Zsanett E-Sólyom Zoltán Kisvárday Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.