Abstract
Surface stress changes associated with hybridization of surface immobilized DNA molecules are measured. A novel interferometry technique employing two adjacent micromachined cantilevers a sensing/reference pair is used to measure the surface stress development. Measurement approach relies on coating the sensing cantilever with single stranded DNA (30-mer Adenine (poly A)) and reference cantilever with hybridized DNA. The sensing/reference pair is exposed to varying concentrations of complimentary DNA strand (30-mer Thymine (Poly T)) and differential deflection of sensing cantilever with respect to reference is measured to determine the change in surface stress. Experimental results indicate that surface stress develops only on exposure to complimentary strands (specific binding) and is not affected by exposure to other single stranded DNA (non-specific binding). Varying the poly T concentration from 0.1 to 1.0 μM results in a linear increase of the surface stress changes from 40 to 110 mN/m during DNA hybridization. Surface stress development starts as soon as the complementary poly T is introduced in the fluid cell and reaches a stable value within 15 minutes of injection time.
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