Abstract
The surface plasmon resonance (SPR) effect was exploited to develop a detector responding to changes in the refractive index for use in high-performance liquid chromatography. The performance of the SPR detector was compared with that of a refractive index (RI) detector from Hewlett-Packard. The effects of the optical fibre connections and light-emitting diode wavelengths (1300 and 850 nm) were studied. The detectors that were tested provided similar limits of detection for the sugars, fructose (7 μg for RI and 5 μg for SPR), glucose (7 μg for RI and 13 μg for SPR) and sucrose (13 μg for RI and 22 μg for SPR). The SPR was found to reach a stable baseline quicker than the RI detector, i.e., 50 min before the RI detector did.
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