Supramolecular assembly between adenocard and native beta-cyclodextrin: Preparation, characterization and in-vitro cytotoxic evaluation

Abstract

The inclusion complexation behavior, characterization and binding ability of adenocard with beta-cyclodextrin were investigated in both solution and the solid state. Absorbance and fluorescence intensities of adenocard were enhanced due to the formation of a 1:1 inclusion complex with beta-cyclodextrin. The inclusion complexed product in solid state was further characterized by Fourier-transform infrared spectroscopy, Scanning electron microscopic images, powder X-ray diffraction pattern, thermo gravimetric/differential scanning calorimetric and rotating-frame overhauser effect spectroscopic techniques. The implementation of molecular docking study confirmed the complexation could reduce the energy of the system. A mechanism was proposed to explain the mode of inclusion in the inclusion process. The cell viability analysis demonstrate that all the inclusion complex are nontoxic against Breast Adenocarcinoma-7 cell lines up to the concentrations 500 µg/mL with contact time of up to 72 hr, whereas beta-cyclodextrin showed the half maximal inhibitory concentration at the concentration of 300 µg/mL.