Spectral Studies on the Supramolecular Assembly of Uridine with β-Cyclodextrin and Its In Vitro Cytotoxicity

Abstract

The supramolecular interaction between Uridine (UR) and β-cyclodextrin (β-CD) has been investigated in liquid, solid, and virtual state. Noncovalent interaction between UR and β-CD in aqueous medium was confirmed by using absorption, fluorescence emission, and time-resolved fluorescence spectroscopy. The solid inclusion complex was prepared using physical mixing, kneading method, and coprecipitation method and it was characterized using FT-IR, SEM, powder X-ray diffraction patterns TG/DSC, and 1H NMR techniques. The stoichiometric ratio of the inclusion complex was found to be 1:1 and the binding constant of the inclusion complex at 303 K were determined using Benesi–Hildebrand plot. The changed in the Gibbs free energy for the complexation reaction was determined and it was found that the complexation process was spontaneous. As the changes in the chemical shift, values of the UR have been observed after the formation of an inclusion complex. The inclusion process is virtually evident by molecular docking studies using the patch-dock server. As the biological application of this complexation, the cytotoxic activity has also been successfully tested against breast cancer cell line which exposed at different concentration for 74 h.