Sucrose and coumarin effect on the growth and development of micro-cutting potato plant (Solanum tuberosum) variety Granola Kembang

Abstract

Abstract. Manjaswari A, Pitoyo A, Sari SLA. 2018. Sucrose and coumarin effect on the growth and development of micro-cutting potato plant (Solanum tuberosum) variety Granola Kembang. Cell Biol Dev 2: 55-62. This research aimed to determine the effect of various concentrations of sucrose and coumarin on the micro-cutting potato plant's growth and development. A completely Randomized Design (CRD) with a single concentration combination factor of sucrose and coumarin was treated well. Five levels of sucrose concentrations were 0, 30, 50, 70, and 90 g/L. While coumarin concentrations were 0, 20, 40, 60, 80 mg/L. Observation and data collection was made in the second month after the explant planting. Some parameters observed were morphological changes in explant characteristics, i.e., the percentage of explants response, the emergence of the shoot, shoot height, shoot number, leaf emergence time, number of leaves, the emergence of the root, root number, and root length. The data obtained were analyzed using ANOVA followed by DMRT at level test 5 % to determine if there is a significant difference between the treatments. The result showed that the higher sucrose concentration added into the media could increase the micro-cut growth and development of shoots and leaves; otherwise, it would negatively influence root development. The addition of coumarin in high concentration inhibited all the parameters of growth and development observed. Both combinations of sucrose and coumarin could increase the micro-cut development of shoot at 50 g/L of sucrose and 20 mg/L of coumarin concentration. Those concentrations could produce the best amount and higher shoot for micro-cut multiplications.