The effects of different concentration of sucrose and various auxin on in vitro shoot and microtuber formation of red potato (Solanum tuberosum, L. var Desiree)

Abstract

The experiment was aims to study the effect of different sucrose concentrations (30, 90, 120 and 150 g.L−1) and various types of auxin (IAA, IBA, NAA) on in vitro shoot and microtuber formation of Red Potato (Solanum tuberosum, L. var Desiree). Young stem cutting from planlet cultured on half strength MS medium. All types of auxin use the concentration of 0.25 mg.L−1. The research conducted a completely randomized design (CRD) factorial. The data obtained were analyzed by analysis of variance (ANOVA) and DMRT test at 0.05. Parameters observed included growth of microtuber and shoot growth, such as number of shoots and shoot length. The observations result for 3 months, show that the higher the concentration of sucrose, can stimulate tuber formation but suppress shoot growth. Early tuber initiation (36,7 DAP) and optimum number of tuber was observed in 150 g.L−1 sucrose, whereas at 30g.L−1 had no effect to tuber induction. In addition, high number of microtuber was observed in NAA compare to other auxin. Observational data on shoot growth showed that the higher concentration of sucrose will inhibit bud formation. The 90 g.L−1 sucrose concentration showed the best number of shoots, which averaged 1.5 shoots/plantlet. But at a concentration of 30g/L sucrose, explants are only able to form callus. In the bud length parameter, IAA gave the best response that is an average of 3cm/shoot.