Abstract
In this work the SERS total half bandwidths of five genomic DNAs fromin vitro-grown apple leaf tissues (Malus domesticaBorkh., FamRosaceae, cvs. Florina, Rebra, Goldrush, Romus 3 and Romus 4) have been measured. We have shown that surface-enhanced Raman scattering can be used to study the fast subpicosecond dynamics of DNA in the proximity of a metallic surface. The dependencies of the total half bandwidths and of the global relaxation times, on DNA molecular subgroup structure, on the type of genomic apple plant DNA and on time, are reported. An example of the time-dependence of SERS band parameters of DNA, in the proximity of silver nanoparticles, has been given. It is shown that changes in the subpicosecond surface dynamics of molecular subgroups in genomic DNAs fromin vitro-grown apple leaf tissues can be monitored with surface-enhanced Raman spectroscopy. Particularly, the SERS band parameters for the vibrations near 757 cm–1(dT), 773 cm–1(dC), 929 cm–1(deoxyribose), 1087 cm–1(PO2–symmetric stretch, backbone), 1127 cm–1(dA), 1181 cm–1(ring vibrations of dG, dT, dC), 1311 cm–1(dA), 1362 cm–1(dT, dA), 1510 cm–1(dA), 1573 cm–1(dG, dA) and 1650 cm–1[dT(C═O), δ(H2O)] of genomic DNAs from apple leaves are presented. In our study, the full widths at half-maximum (FWHM) of the bands in genomic DNAs fromin vitro-grown apple leaf tissues are typically in the wavenumber range from 14 to 52 cm–1. Besides, it can be observed that molecular relaxation processes studied in this work, have a global relaxation time smaller than 0.76 ps and larger than 0.20 ps. A comparison between different ranges of FT-Raman and SERS band parameters of DNA extracted from leaf tissues, respectively, is given. We have found that the bands of DNA from Romus 3 and Rebra cultivars are suitable for studying the dynamical behaviour of molecular subgroups, in genomic nucleic acids extracted fromin vitro-grown apple plants.
Highlights
Biological world is one of perpetual movement, a fundamental characteristic of life
We have found that the bands of DNA from Romus 3 and Rebra cultivars are suitable for studying the dynamical behaviour of molecular subgroups, in genomic nucleic acids extracted from in vitro-grown apple plants
It is shown that changes in the subpicosecond dynamics of molecular subgroups in genomic DNAs from in vitro-grown apple leaf tissues, in the proximity of a silver surface, can be monitored with surfaceenhanced Raman spectroscopy (SERS)
Summary
Biological world is one of perpetual movement, a fundamental characteristic of life. The dynamics of macromolecules is a fundamental component of their behaviour. A confocal Raman microspectroscopic study into the vibrational half bandwidths of molecular subgroups in calf-thymus DNA at pH 7 and pH 3, in the presence of Na+, Ca2+ and Mg2+ ions, respectively, was previously reported by us [6]. The dependencies of the full widths at half-maximum (FWHM) and of the global relaxation times on DNA molecular subgroup structure and on pH values, in the presence of Mn2+ ions, have been studied [8]. Monitoring the changes in the Raman full widths at half-maximum (FWHM) and, correspondingly, in the global relaxation times of the molecular subgroups in DNA, upon varying the Zn2+ concentration, was of interest. It is shown that changes in the subpicosecond dynamics of molecular subgroups in genomic DNAs from in vitro-grown apple leaf tissues, in the proximity of a silver surface, can be monitored with surfaceenhanced Raman spectroscopy (SERS). A FT-Raman study of the (sub)picoseconds dynamics in genomic DNA from plant tissues was done by us [4] and is used for comparison in the present work
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