Abstract
In this work the SERS total half bandwidths of six genomic DNAs from ex vitro-grown apple leaf tissues (Malus domesticaBorkh., Fam. Rosaceae, cvs. Rebra, Goldrush, Florina, Romus 4, and the rootstocks M26, M9) and of five genomic DNAs fromin vitrogrown plants ofArnica montana(L.), Fam. Compositae,Astragalus peterfii(Jáv.), Fam. Fabaceae, strawberry (Duch.), Fam. Rosaceae, carnation (Dianthus caryophyllusL.), Fam. Caryophyllaceae, andKalanchoe x hybrida, Fam. Crassulaceae, respectively, have been measured. We have shown that surface-enhanced Raman scattering can be used to study the fast subpicosecond dynamics of DNA in the proximity of a metallic surface. The dependencies of the total half bandwidths and of the global relaxation times, on DNA molecular subgroup structure and on the type of genomic DNA, are reported.It is shown that changes in the subpicosecond surface dynamics of molecular subgroups in genomic DNAs from leaf tissues can be monitored with surface-enhanced Raman spectroscopy. In our study, the full widths at half-maximum (FWHMs) for the SERS bands of genomic DNAs from different leaf tissues, are typically in the wavenumber range from 15 to 30 cm–1for data set 1 and from 13 to 42 cm–1for data set 2. Besides, it can be observed that molecular relaxation processes studied in this work, have a global relaxation time smaller than 0.71 ps and larger than 0.35 ps (data set 1) and also varying between 0.25–0.82 ps for data set 2. A comparison between different ranges of FT-Raman and SERS band parameters, respectively, of DNA extracted from leaf tissues is given. We have found that the bands of DNA from Florina cultivar and Kalanchoe leaves, respectively, are suitable for the study of dynamical behaviour of molecular subgroups in nucleic acids extracted from different leaf tissues.
Highlights
Studies of relaxation processes in liquids are valuable in providing information about the intermolecular interactions in condensed matter [3]
We have shown that surface-enhanced Raman scattering can be used to study the fast subpicosecond dynamics of DNA in the proximity of a metallic surface
We have found that the bands of DNA from Florina cultivar and Kalanchoe leaves, respectively, are suitable for the study of dynamical behaviour of molecular subgroups in nucleic acids extracted from different leaf tissues
Summary
Studies of relaxation processes in liquids are valuable in providing information about the intermolecular interactions in condensed matter [3]. The dependencies of the full widths at half-maximum (FWHMs) and of the global relaxation times on DNA molecular subgroup structure and on pH values, in the presence of Mn2+ ions, have been studied [7]. Monitoring the changes in the Raman full widths at half-maximum (FWHMs) and, correspondingly, in the global relaxation times of the molecular subgroups in DNA, upon varying the Zn2+ concentration, was of interest. In a previous study of us, the full widths at half-maximum (FWHMs) of the SERS bands in genomic DNAs from in vitro-grown apple leaf tissues were typically in the wavenumber range from 14 cm−1 to 52 cm−1. It is shown that changes in the subpicosecond dynamics of molecular subgroups in genomic DNAs from different leaf tissues, in the proximity of a silver surface, can be monitored with surface-enhanced Raman spectroscopy (SERS). A FT-Raman study of the (sub)picosecond dynamics in genomic DNA from plant tissues was done by us [8] and is used for comparison in the present work [9]
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