Abstract
Erythrina variegata is a herbal plant commonly used in Indonesia traditional medicine for treatment malaria disease. Acute toxicity test results showed that the methanol extract of E. variegata leaves categorized as practically nontoxic. In this study, we investigated sub chronic toxicity of the extract on male Wistar rats and the procedure based on guidelines of OECD 408 (1998) and EPA OPPTS 870.3100 (1998) for 90 constitutive days.  The rats are classified into four dose groups were 0 (control), 250, 500, and 1000 mg/kg body weight with each group consist of five test animals. The results do not show toxic signs either behavior or body weight changed. Hematological analysis and clinical blood chemistry revealed slightly changes but were within the normal limits, except for BUN and SGPT values. Histopathological examination showed an increased damage of liver and kidney cells in form hydropic degeneration, fatty degeneration, as well as necrosis along with increased of extract doses that significantly different with control (p<0.05). However, the damage is reversible and was assumed had not has relations with the treatment. Thus, orally administration of E. variegata extract by dosage 250, 500, and 1000 mg/kg BW did not cause subchronic toxicity on male Wistar rats.       Â
Highlights
We investigated the subchronic toxicity of the extract on male Wistar rats and the procedure based on guidelines of OECD 408 (1998) and Environmental Protection Agency (EPA) OPPTS 870.3100 (1998) for 90 constitutive days
The objective of this study was to investigate the subchronic toxicity of methanol extract from E. variegata leaves in male Wistar rats
The subchronic oral administration of methanol extract of E. variegata leaves at a doses 250, 500, and 1.000 mg/kg B.W resulted in neither change in behavior nor toxic signs during the experimental period as shown by the normal rate of respiration, the color of body surfaces, body posture, movement, and reflex
Summary
E. indica) belonging to the family Leguminosae known as Indian Coral tree or Tropical coral tree or Variegated coral tree, is a thorny deciduous tree growing to 60 feet tall, and is the most attractive type of genus Erythrina because of its variegated leaves, and its seasonal showy red flowers that spread broadly open and creates a broad canopy, but has spiny branches (Kumar et al, 2010; Suryawanshi & Patel, 2011). Subchronic Toxicity of Methanol Extract from Erythrina variegata. Of the plant have been used in traditional medicine, for instance in Indian preparation is used in many traditional medicines, including to destroy pathogenic parasites and relieve joint pain (Suryawanshi & Patel, 2011). Malaria is a life-threatening disease caused by protozoan parasites from the Plasmodium family which can be spread through Anopheles mosquito bite or by a contaminated needle or transfusion.
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