Abstract
Meniran (Phyllantus niruri L.) and Pegagan (Centella asiatica L.) was proven had activity that considered as an antioxidant. However, the safety was not confirmed. This research is intended to evaluate sub-chronic toxicity of standardized combination extract of meniran and pegagan on liver and kidney function. This research used post-test-control design. Twenty female and twenty male Wistar strain rats divided into 4 groups. The first group was controlled in dosage of CMC Na 0.5%. The others were given a combination of meniran and pegagan extract in ratio with different dosage (50:50; 250:250; and 1250:1250 mg/KgBW). Sub-chronic toxicity test of meniran and pegagan combination was given orally once a day for 28 consecutive days. On the 29th day, all the rats were sacrificed and blood samples were analyzed using automatic analyzer. SGOT, SGPT, BUN, and creatinine value were statistically analyzed using one way ANOVA with post hoc LSD (p<0.05). The results show that treatment of meniran and gotu kola combination had no significantly different of SGOT and creatinine value of male and female rats, and SGPT value of male rats (p>0.05). The treatment of meniran and gotu kola combination had significantly different of BUN value of male and female rats, and SGPT value of female rats (p<0.05) but still within normal range. Based on this study, it can be concluded that orally administered of meniran and gotu kola extract combination at doses 50:50 mg/KgBW; 250:250 mg/KgBW; and 1250:1250 mg/KgBW had no affected on SGOT, SGPT, BUN and creatinine value of Wistar strain rats on sub-chronic administration for 28 consecutive days.
Highlights
Meniran (Phyllantus niruri L.) and Pegagan (Centella asiatica L.) was proven had activity that considered as an antioxidant
Research performed by Praptiwi (2010) argued that saponin substance is main component of pegagan extract that can cause hemolysis and decrease surface pressure, so that it can result degeneration and sinusoid congestion, color changing and there is white spot in liver caused by cell destruction and leucocyte accumulation
Sub-chronic toxicity testing of meniran extract at dosage of 2.000 mg/KgBW and 5.000 mg/KgBW showed that there is no significant difference against the content of serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) of rats after 14 days of administering (Asare et al, 2011)
Summary
Meniran (Phyllantus niruri L.) and Pegagan (Centella asiatica L.) was proven had activity that considered as an antioxidant. It can be concluded that orally administered of meniran and gotu kola extract combination at doses 50:50 mg/KgBW; 250:250 mg/KgBW; and 1250:1250 mg/KgBW had no affected on SGOT, SGPT, BUN and creatinine value of Wistar strain rats on sub-chronic administration for 28 consecutive days. Sub-chronic toxicity testing of meniran extract at dosage of 2.000 mg/KgBW and 5.000 mg/KgBW showed that there is no significant difference against the content of serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) of rats after 14 days of administering (Asare et al, 2011). Sujono et al (2015) stated that administering of ethanol-meniran extract at dosage of 50 mg/KgBW and 250 mg/KgBW does not cause significant difference against decreasing SGPT level measured in the 0-day and 90-day at Sprague Dawley strain rats. Another research depicted that repeated dosage of pegagan extract at 9.07 mg/20 grams to mice or equivalent to 63.49 mg/200 grams of rats can cause changes of liver and kidney (Praptiwi, 2010)
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