Abstract
The aim of the study was to clarify the role of LGR5 in the process of formation and development of gastric cancer. The expression of LGR5 in gastric cancer and corresponding non-cancerous gastric tissues of 52 gastric cancer patients was assessed with the real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and immunohistochemistry. We also analyzed the relationship between the expression level and clinicopathological characteristics. LGR5 gene and protein expression in gastric cancer tissues was in both cases significantly higher than in corresponding non-cancerous gastric tissues (both P 0.05, respectively). Furthermore, LGR5 gene expression was markedly higher in gastric cancer tissues of Helicobacter pylori (HP)-positive patients than negative ones (P < 0.05). The result of the study showed that LGR5 might play a significant role in the process of formation and development of gastric cancer as an oncogene. Its effect might be strengthened by HP infection.
Highlights
Leucine rich repeat containing G-protein-coupled receptor 5 (LGR5) gene and protein expression in gastric cancer tissues was in both cases significantly higher than in corresponding non-cancerous gastric tissues, but no significant relationship was found with clinicopathological parameters including tumor location, depth of invasion, differentiation, lymph node metastasis, stage, gender, age and carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA19-9) level in peripheral blood preoperation of patients (P > 0.05, respectively)
LGR5 is the target gene of Wnt signaling pathway, which is involved in the formation of stem cell signaling network, and related to the occurrence and development of tumor [5]
The human LGR5 gene is located on the chromosome 12q22-23 with a total length of 4208 bp, containing 22 exons and 21 introns
Summary
Tumor stem cells doctrine that tumor stem cells are primary cell lines in a class of self-renewal, infinite proliferation and multi-directional differentiation of cell subsets, is the tumor growth, metastasis, recurrence of the root causes. Compared with the corresponding normal tissue, LGR5 is over expressed in human primary colon cancer, ovarian cancer, hepatocellular carcinoma [3] [4], suggesting that there is a certain correlation between LGR5 and tumor. LGR5 is the target gene of Wnt signaling pathway, which is involved in the formation of stem cell signaling network, and related to the occurrence and development of tumor [5]. The real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry were used to detect the expression of LGR5 in gastric cancer tissues in this study, and to explore the correlation between LGR5 and clinicopathological parameters, and to study the relationship between LGR5 and gastric cancer proliferation, to determine whether the LGR5 gene is a prognostic and recurrence of speculation indicators
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