Ubiquitin Fusion Degradation 1 like Protein Expression in Gastric Cancer and its Clinical Significance

Abstract

To investigate the expression of ubiquitin fusion degradation 1 like protein in gastric cancer and its relationship with the clinicopathological characteristics. Analyzed the data of ubiquitin fusion degradation 1 like protein in gastric cancer and normal tissues in the Cancer Genome Atlas database and the University of Alabama Cancer database. The expression of ubiquitin fusion degradation 1 like protein from fresh samples was detected by real-time quantitative polymerase chain reaction and Western blot, the expression of ubiquitin fusion degradation 1 like protein from paraffin samples was detected by immunohistochemistry. Observed the relationship between the expression level of ubiquitin fusion degradation 1 like protein and clinicopathological characteristics of patients. Data from the Cancer Genome Atlas and the University of Alabama Cancer Databases suggested that ubiquitin fusion degradation 1 like protein messenger ribonucleic acid expression in gastric cancer tissues was significantly higher than that in normal gastric tissues (p<0.05); Western blot and real-time fluorescence quantitative polymerase chain reaction confirmed that ubiquitin fusion degradation 1 like protein and messenger ribonucleic acid expression in fresh gastric cancer tissues were significantly higher in adjacent tissues (p<0.05). Immunohistochemistry results showed that 88 cases of gastric cancer had positive protein expression in 71 cases and negative expression in 17 cases. The expression difference of ubiquitin fusion degradation 1 like protein in gastric cancer and non-tumor tissues adjacent to the cancer was statistically significant (p<0.05) and it was significantly correlated with tumor, nodes and metastases stage and differentiation degree (p<0.05). The expression level of ubiquitin fusion degradation 1 like protein in gastric cancer tissues is significantly increasing and associated with disease progression, suggesting ubiquitin fusion degradation 1 like protein may be involved in the development and progression of gastric cancer.