Study on the Predominant Bacteria from Storage of Liquid-Smoked Tilapia

Abstract

16SrDNA PCR-DGGE was used to reveal the dynamics of bacterial colony and identify the predominant bacteria in liquid-smoked tilapia during storage at 25°C. Total bacteria DNA was directly extracted from the tilapia fillets. By analysis of the community changes directly obtained from the DGGE profiles, the predominant bacteria were found to bePediococcus pentosaceus,Clostridium sp.andLactococcusgarvieae. Simultaneously, culture method was performed., 34 strains of 70 colonies were picked up from samples of day 4 storage period, then 6 strains were picked up at random and identified by extracting their 16SrDNA and sequencing, it’s proved that they were allLactococcus. So Two ways of identifying the predominant bacteria were almost consistent.