Abstract
ABSTRACTPurposeTo study the Periplaneta americana L. extract Ento-B on the treatment of chronic ulcerative colitis induced by 2,4-dinitrochlorobenzene and acetic acid in rats and to explore its primary mechanism of action.MethodsUsing 2,4-dinitrochlorobenzene combined with acetic acid to induce chronic ulcerative colitis (chronic UC) in rats. The sulfasalazine (400 mg/kg) and Ento-B (200 mg/kg, 100 mg/kg,50 mg/kg) were given by intragastric administration and the effect was evaluated according to the disease activity index (DAI) score, colon mucosal injury index (CMDI) score, histopathological score (HS) and the serum levels of Interleukin-4(IL-4), Interleukin-10(IL-10), Tumor necrosis factor-α(TNF-α), Malondialdehyde(MDA), Superoxide dismutase(SOD) and Inducible nitric oxide synthase(iNOS.)ResultsCompared with the model group, all doses of Ento-B could reduce the score of CMDI (p < 0.05), HS(p < 0.05 or p < 0.01), significantly increased the expression of IL-4, IL-10, SOD (p < 0.01) and decreased the levels of TNF-α, MDA, iNOS in serum of UC rats, significantly improving the degree of colon lesionsin UC rats.ConclusionsEnto-B may play an important role in the treatment of ulcerative colitis induced byUC rats. The mechanism may be related to the increased expression of IL-4, IL-10, SOD and reduced expression of TNF-α, MDA, iNOS.
Highlights
Ulcerative colitis (UC) is a common chronic nonspecific intestinal disease with unknown pathogenesis
The rats in the model group showed varying degrees of mental malaise, loose, yellowish, dull hair, slow reaction, rapid weight loss, a significant reduction in food intake, arched back, gathering and lazy movement. It is accompanied by symptoms such as bloody stool or diarrhea, feces adhesion to the anus and so on
One week after the end of the model, the mental state of the rats recovered to a certain extent, sparse stool, blood in the stool and weight gain; after treatment with SASP, the mental state, independent movement ability and bodyweight of UC rats recovered quickly and the number of stools decreased, the sparse stool disappeared and the feces formed
Summary
Ulcerative colitis (UC) is a common chronic nonspecific intestinal disease with unknown pathogenesis. Aminosalicylic acid, glucocorticoid and immunobiological treatment are mainly used in clinics, but the use is limited because of unstable curative effect, high toxicity and strong dependencey[2]. The previous study of the group found that the active ingredient Ento-B extracted from Periplaneta americana L. by new technology has the effect of promoting coagulation and hemostasis[10]. The main purpose of this study was to investigate the effect of Ento-B on ulcerative colitis induced by 2,4-dinitrochlorobenzene (DNCB) combined with acetic acid in rats and to explore its mechanism, so as to provide effective data support for the development of Periplaneta americana L. extract Ento-B as a drug for the prevention and treatment of ulcerative colitis
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