Study on the chiroptical properties of carbon dots based on citric acid and formamide with the addition of various chiral molecules

Abstract

Subject of study. The study focuses on luminescent carbon nanoparticles, specifically carbon dots, based on citric acid, formamide, and various chiral molecules. Aim of study. The aim is to assess the influence of chiral molecules used in the synthesis of carbon dots on optical transitions in the long-wavelength spectral region and to investigate the mechanism underlying the circular dichroism signal of these nanoparticles. Method. Carbon dot samples were synthesized using two methods: (i) a one-step solvothermal synthesis of carbon dots from citric acid, formamide, and various chiral molecules and (ii) a two-step method involving the solvothermal synthesis of achiral carbon dots from citric acid and formamide, followed by surface treatment with L-cysteine. Absorption and luminescence spectroscopy methods were employed to study the chemical structure and optical properties of the carbon dots. The chiroptical properties of the obtained samples were analyzed using circular dichroism spectroscopy. Main results. The addition of various chiral molecules to a mixture of precursors during one-step synthesis enabled the fabrication of carbon dots with different chemical compositions, including variations in surface groups and types of emission centers in the spectral region of 350–700 nm. The use of L-phenylglycine and L-tryptophan in the one-step synthesis was shown to lead to the formation of nanoparticles with optical transitions in both short- and long-wavelength regions of the spectrum. The addition of L-glutathione during the one-step synthesis resulted in the formation of carbon dots with short-wavelength emission, while the addition of L-cysteine did not alter the emission characteristics of achiral carbon dots based on citric acid and formamide. The optical properties of the chiral carbon dots obtained using the two-step synthesis method with L-cysteine remained unchanged compared with those of the achiral carbon dots synthesized from citric acid and formamide. The circular dichroism spectra of all the samples showed a signal at ∼250nm, which was attributed to the derivatives of the chiral precursors attached to the surface of the nanoparticles, irrespective of the preparation method. Practical significance. Chiral carbon dots hold promise in biomedicine as sensors, luminescent biomarkers, and other applications due to their biocompatibility and non-toxicity. The results obtained in this work will serve as a foundation for the further fabrication and investigation of chiral carbon nanoparticles with long-wavelength luminescence.