Abstract
Objective To predict the main active ingredients, potential targets, and key pathways of Jiawei Chaiqin Wendan decoction treatment in vestibular migraine and explore possible mechanisms by network pharmacology and molecular docking technology. Methods The active ingredients and related targets of Jiawei Chaiqin Wendan decoction were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The corresponding genes of the target were queried by UniProt database, and the “drug-compound-target-disease” network was constructed by Cytoscape 3.7.2 software. GO functional enrichment analysis and KEGG pathway enrichment analysis were carried out by R software and Bioconductor, and column chart and bubble chart were drawn by Prism software and OmicShare database for visualization. Finally, the mechanism and potential targets of Jiawei Chaiqin Wendan decoction in the treatment of vestibular migraine were predicted. Results The “drug-compound-target-disease” network contains 154 active ingredients and 85 intersection targets. The key targets include AKT1, IL6, MAPK3, VEGFA, EGFR, CASP3, EGF, MAPK1, PTGS2, and ESR1. A total of 1939 items were obtained by GO functional enrichment analysis (P < 0.05). KEGG pathway enrichment analysis screened 156 signal pathways (P < 0.05), involving PI3K-Akt signal pathway, AGE-RAGE signal pathway in diabetes complications, MAPK signal pathway, HIF-1 signal pathway, IL-17 signal pathway, etc. Molecular docking results showed that quercetin, luteolin, kaempferol, tanshinone IIa, wogonin, naringenin, nobiletin, dihydrotanshinlactone, beta-sitosterol, and salviolone have good affinity with core target proteins IL6, PTGS2, MAPK1, MAPK3, and CGRP1. Conclusion The active ingredients in Jiawei Chaiqin Wendan decoction may regulate the levels of inflammatory factors and neurotransmitters by acting on multiple targets such as IL6, MAPK3, MAPK1, and PTGS2, so as to play a therapeutic role in vestibular migraine.
Highlights
Vestibular migraine (VM) is a chronic and disabling disease that is common and has a genetic tendency; the main symptoms are recurrent dizziness/vertigo, which may be accompanied by nausea and vomiting with or without headache [1]
Based on TCMSP and DrugBank database, a total of 3376 protein targets related to the above active ingredients were obtained by Perl software, and 1531 gene symbols were obtained after gene annotation using UniProt KB
It has been found that there is a close connection between the nervous system and the immune system, and inhibiting the expression level of inflammatory factors can reduce the symptoms of VM or the occurrence of VM [13]
Summary
Vestibular migraine (VM) is a chronic and disabling disease that is common and has a genetic tendency; the main symptoms are recurrent dizziness/vertigo, which may be accompanied by nausea and vomiting with or without headache [1]. Studies have shown that VM is one of the common causes of recurrent dizziness or vertigo [1,2]. Trigeminal neurovascular system has been widely regarded as the basis of this highly complex nervous system disease for 40 years [4]. CSD activates the trigeminal neurovascular system, and the trigeminal ganglion releases calcitonin gene-related peptides (CGRP), Evidence-Based Complementary and Alternative Medicine substance P, and other neuropeptides, causing meningeal vascular inflammation, and leading to migraine symptoms. The overlap of the pathways responsible for pain and balance in the central nervous system, the fibrous connection between the trigeminal nucleus and the vestibular nucleus, and the trigeminal nerve innervating the inner ear eventually lead to vestibular symptoms [5]
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