Study on the molecular mechanism of Huang jing in the treatment of type 2 diabetes mellitus based on network pharmacology

Abstract

Objective To explore the possible mechanism of Huang jing in the treatment of T2DM by using network pharmacology. Methods Using TCMSP database to obtain the active components and targets of Xanthine, combined with OMIM, GeneCards, DrugBank, DisGeNET and other databases to screen T2DM-related gene targets, extracting drug-disease target intersection with R software, and constructing drug-component-disease-target network diagram. The intersection target PPI network is constructed through the String database and Cytoscape software. The GO function and KEGG pathway enrichment analysis of the target of T2DM are performed using R software and Metascape database. Results 12 active ingredients and 76 targets are selected from Huang jing. Key proteins involved AKT1, VEGFA, PPARG, CASP3, FOS, MAPK14, MMP9, etc. 818 items are obtained by GO functional enrichment analysis, KEGG pathway enrichment screening obtained 80 signaling pathways is mainly concerned with the signaling pathways including whereas resistance, age-rage signaling pathway in the diabetic outcome, MAPK signaling pathway, and Relaxin signaling pathway. Conclusion The possible mechanism of action of xanthine in the treatment of T2DM is predicted by using network pharmacology method. The useful components of Xanthine mainly coordinated the intervention of T2DM by coordinating inflammatory signaling pathway, insulin-related pathway and antioxidation-related signaling pathway, providing new ideas and clues for further clinical application research.