Study on applications of rheum sterile solution in rats following sepsis and effect on inflammatory factors

Abstract

Objective To study the therapeutic effect of rheum(Chinese herbal medicine) preparation made by using ultrasonic technique on pro-inflammatory cytokines and sepsis in rats. In order to offer novel measure for the treatment of critically ill patients. Methods Firstly, rheum sterile solution was prepared through ultrasonic technique. Secondly, fifty healthy male SD rats were randomly(random number) divided to CLP group and rheum group. Moderate degree of sepsis model was established by using cecal ligation and puncture(CLP). Rats in group rheum received the liquid rheumpreparation via intragastric administration, while rats in group CLP received saline instead. The 7-day survival rate was recorded and was compared between two groups. In addition, another fifty-four rats were randomly(random number) divided to sham group, CLP group and rheum group(n=18 in each group). CLP was performed to induce sepsis in CLP group and rheum group. Then rats in rheum group received rheum sterile solution via intragastric administration, while rats in CLP group received saline instead. At 12 hours, 24 hours and 48 hours after modeling, six rats in each group were randomly sacrificed. Serum TNF-α and HMGB1 levels were detected by ELISA method. Levels of RAGE, HMGB1 and NF-κB P65 in small intestine were detected by Western Blot. Results Level of anthraquinones extracted from rheum by ultrasonic technique was higher than that by conwentional decoction method. The 7-day survival rate of rats in rheum group(76%) was higher than that in CLP group(48%)(P 0.05). At 24 hours and 48 hours after modeling, serum HMGB1 levels were significantly lower in rheum group than those in CLP group(P<0.05). Compared with sham group, protein levels of HMGB1, RAGE and NF-κB in small intestine were elevated in CLP group and rheum group at 48 hours after modeling(P<0.01), while protein levels of above biomarker were higher in CLP group than those in rheum group(P<0.05). Conclusions Rheum sterile solution could down-regulate the level of pro-inflammatory cytokines, modulate the inflammatory response, and improve the survival rate in rats with sepsis. Key words: Ultrasonic technique; Rheum sterile solution; Stability; Sepsis; Proinflammatory cytokines