The effect of continuous infusion of lidocaine on high mobility group box 1 expression in lung tissue of sepsis model rats

Abstract

Objective To evaluate the effect and mechanism of intravenous infusion of lidocaine on the expression of high mobility group box 1 (HMGB1) mRNA in lung of sepsis model rats. Methods Senenty-five healthy male Sprague-Dawley (SD) rats were stochastically divided into 5 groups (n=15): S group (group S), cecal ligation and puncture (CLP) group (group CLP), pre-CLP infusion the lidocaine group (group L1), CLP immediately infused with lidocaine (group L2) and CLP infused with the lidocaine-treated group (group L3). Group S suture abdominal cavity after opening it while other groups were ligated with cecal ligation and puncture. groups L1, L2 and L3 were infused with lidocaine for 3 h before CLP, at the moment of CLP started and 3 h after CLP was completed respectively. The group S and the group CLP were replaced by the same amount of normal saline. The rats were sacrificed 24 h after the CLP model was successfully established. The serum levels of pro-inflammatory cytokines IL-6, TNF-α and HMGB1 concentration were measured by enzyme-limked immunosorbent assay(ELISA). The expression of HMGB1 mRNA in lung tissue was detected by qRT-PCR. Automated biochemical analysis was used to detect serum ALT, AST, and Cr. Another 50 rats were grouped and observed for 72 h. Results Compared with group S, CLP occurred in the group CLP, group L1, group L2 and group L3, IL-6, TNF-α, HMGB1 concentrations and the expression of HMGB1 mRNA were increased in lung tissues (P<0.05). The serum levels of HMGB1, IL-6, TNF-α and HMGB1 mRNA in lung tissues of the groups L1, L2 and L3 were significantly lower than those in the group CLP (P<0.05). Compared with group S, ALT, AST and Cr were increased in groups CLP, L1, L2, and L3(P<0.05). Compared with group CLP, ALT, AST and Cr were decreased in groups L1, L2 and L3(P<0.05). Compared with the group CLP, the survival rates of the groups L1, L2 and L3 were significantly improved. Conclusions CLP can increase the expression levels of pro-inflammatory cytokines in rats. Intravenous infusion of lidocaine can effectively reduce the expression of inflammatory factors in CLP rats and inhibit the expression of HMGB1 mRNA in lung tissues. Thus, it reduces the lung damage caused by CLP and improves the survival rate effectively. Key words: Lidocaine; Sepsis; High mobility group box 1