Study of some toxicological aspects of Alfa-cypermethrin in rabbits

Abstract

This study was conducted to evaluate the immediate alterations that occur in rabbitsafter acute toxicity with single oral dose of cypermethrin. Twenty one rabbits were randomlydivided into three equal groups: treated groups (A1), (A2) and control group used in thisstudy. Groups (A1), (A2) received orally cypermethrin in dose of (25, 50 mg/kg body weight)respectively, While control group received normal saline at same time. Most obvious clinicalsings in the treated groups were Restlessness, salivation, licking of legs and face, frequenturination, muscular tremor, incoordination, and ataxia appeared in dose dependent manner. Inaddition, significantly increased in respiratory rate and heart rate in treated groups comparedwith control group and appeared petechial hemorrhage and congestion in the conjunctiva intreated groups. The blood samples were taking to evaluate some of blood parameters includes(RBC counts, WBC counts, PLT counts, Hb concentration, PCV, RBCs indices (MCV, MCH,MCHC, RDW -CV and RDW - SD), PDW, MPV, P-LCR and some biochemical study asALT and AST. Two rabbits from each group were killed to evaluate the histopathologicalalteration in liver. The statistical analysis revealed that there were significant decreased inRBC counts and Hb concentration (P<0.05) in treated groups (A1), (A2) as compared withcontrol group. The PCV percentage, platelet counts and MCHC values revealed significantdecreased (P<0.05) between group (A2) and control group. The RDW-SD, RDW-CV valveswere significantly increased (p<0.05) between group (A1) and control group. Other parameterstudied did not show significant differences between treated groups and control group. Thestatistical analysis revealed that the levels of ALT and AST were significantly elevated(P<0.05) in (A1 and A2) groups as compared with control group. In gross appearance therewere petechial hemorrhage and erosion of liver parenchyma. Histopathological study revealedthat there were engorgement of hepatic vein with presence of extravasated RBCs between thehepatocytes in group (A1), while group (A2) the hepatocytes undergo atrophy and severenecrosis and destruction with infiltration of polymorphnuclear leukocytes and engorgement ofsome portal trait with bile secretion