Environmental, Biochemical and Hematological study in rats exposed to Cadmium Chloride in Drinking Water and the role of Vitamin E and C

Abstract

The present study was carried out to determine the synergistic role of vitamin E and vitamin C in reducing the toxic effects of cadmium chloride on some blood parameters (RBC count, Hb concentration, PCV, Total WBC count, Differential WBC count), as well as some biochemical parameters which include (Malondialdehyde (MDA), Aspartate aminotransferase(AST), Alanine aminotransferase (ALT), Alkaline phosphatase(ALP), Creatinin, Urea) and some antioxidants (Superoxidedismutase(SOD), Glutathion-S-transferase(GST), Catalase(CAT). Forty adult male rats were divided into five equal groups. The first group was left without any treatment (the control group), the second was given cadmium chloride (30mg/L), the third was given cadmium chloride and vitamin E simultaneously, the fourth was given cadmium chloride and vitamin C simultaneously, the fifth was given cadmium chloride, vitamin E and vitamin C simultaneously. The results showed a significant decrease (P <0.05) in (RBC count, Hb concentration, PCV) and a significant increase (P <0.05) in WBC count (neutrophils and eosinophils). On the other hand, the results also showed an increase (P <0.05) in the concentration of (ALT, AST, ALP, Urea, Creatinine, and MDA)and a significant decrease in antioxidants(SOD, CAT, GST) in (T1) compared with the control group. On the other hand, the results showed a noticeable improvement in the parameters studied when using vitamin E and vitamin C alone or together with cadmium chloride, and this indicates the therapeutic role played by vitamins in reducing the toxicity caused by cadmium chloride