Abstract
Objective: Type 2 diabetes mellitus is a major risk factor for aortic calcifications and cardiovascular disease (CVD). Matrix Gla proteins (MGP) have a significant role in control of the process of calcification. The purpose of this study was to investigate the role of MGP G-7A and T-138C gene polymorphisms in development of aortic calcification and CVD in patients with type 2 diabetes mellitus. Material and Methods: The study included 120 patients with type 2 diabetes mellitus and 134 control group. The MGP G-7A and T-138C gene polymorphisms were identified using polymerase chain reaction (PCR) and followed by restriction fragment length polymorphism (RFLP) methods. Results: The G-7A genotype distribution in patients with type 2 diabetes mellitus AA=10.8%, GA=41.7% and GG=47.5% did not significantly differ from those in control group AA=15.7%, GA=48.5% and GG=35.8% (P=0.146). The T-138C genotype distribution in patients with type 2 diabetes mellitus CC=8.4%, CT=40.8% and TT=50.8% were also not significantly different from those in control group CC=3.7%, CT=39.6% and TT=56.7% (P=0.259). On the other hand; age, fasting blood glucose (FBG), cholesterol, high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) as expected were significantly differed between the patient-control groups (p<0.05). Conclusion: This patient-control study show that G-7A and T-138C gene polymorphisms of MGP are not genetic risk factors for type 2 diabetes mellitus.
Highlights
Diabetes mellitus is an important health problem due to the illness itself and its life threatening complications
In the control group type 2 diabetes mellitus, ischemic heart disease (IHD), peripheral arterial disease (PAD), stroke and any chronic illness patients were not included in this study
No significant differences were detected between type 2 diabetes mellitus and control groups for TG, systolic blood pressure (SBP), diastolic blood pressure (DBP) (p>0.05)
Summary
Diabetes mellitus is an important health problem due to the illness itself and its life threatening complications. Type 2 diabetes mellitus is a disease characterized by insulin resistance and cell failure leading to elevated blood glucose levels [1]. Aortic calcifications and cardiovascular diseases (CVD) are an important factor in the pathophysiology of type 2 diabetes mellitus [2]. Tissue calcification is expected to be high due to high extracellular fluid concentrations of calcium and phosphate ions [3]. The human MGP gene is located on the short arm of chromosome 12 and comprises 5 exons (12p12.3). We were taken as a reference MGP-004 in our study. (Transcript ID: ENST00000228938)
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