Study of Caffeine-Loaded Gelatin Nanoparticles for Treatment of Melanoma and Fibroblast Cells

Abstract

In this work, we aimed to study the effect of caffeine-loaded gelatin nanoparticles on melanoma cells and fibroblast cells. The B16F10 murine melanoma cells and L929 fibroblast cells were treated with a different dilution ratio of caffeine-loaded gelatin nanoparticles for 24, 48, and 72 h. The cell assay results showed that treatment with caffeine-loaded gelatin nanoparticles (25 % and 50 %) effectively inhibited the proliferation, viability, and migration ability of B16F10 melanoma cells at 48 and 72 h. Moreover, we also found that the cell apoptosis of B16F10 melanoma cells was induced by treatment of 12.5, 25, and 50 % caffeine-loaded gelatin nanoparticles. In the meantime, for L929 fibroblast cells, there was no significant cell cytotoxic effects observed with identical treatment. In summary, the caffeine-loaded gelatin nanoparticles induced apoptotic process inhibited cell viability and migration ability of melanoma cells and could be an alternative therapy for melanoma cancer.