Study of Association of Hematological Parameters with HbA1c and Microvascular Complications in type II Diabetes Mellitus

Abstract

Background: Studies have proved that complications of diabetes particularly the microvascular complications are strongly associated with HbA1c levels [2,3]. The higher the Hba1c values the higher the chance of developing complications. Hba1c values have thus become a valuable marker in assessing the glycemic control and the risk of developing complications as it is convenient, reproducible and efficient in reflecting chronic hyperglycemia. Aim: To assess the association and correlation of haematological parameters with HbA1c levels in type II diabetes mellitus patients. Methodology: A cross-sectional study was conducted for a period of one year with 150 patients with diabetes and 150 controls were taken without diabetes. Patients’, age, gender, blood pressure, accompanying disease history, medication history and medical history were recorded using pre-tested and semi-structured questionnaire. Five milliliters of fasting blood sample was collected by laboratory technologist for fasting blood glucose (FBG) determination after 10–12 hours of fasting with the exception of water and medication. FBG was estimated by following glucose oxidase method using MINDRAY BS300 fully auto chemistry analyzer, made in china according to manufacturer’s instructions. The quantitative urine albumin/creatinine ratio in morning spot urine samples was used for albuminuria determination in the diagnosis of microalbuminuria. Results: The mean values of fasting and postprandial blood glucose along with HbA1c were found to be significantly higher among the diabetes group than the control group, similarly the urine albumin levels also was found to be significantly higher in the diabetes group, whereas the renal parameters (serum urea and creatinine) did not show statistical significant difference between the diabetes and control group. HbA1c and the other hemotological parameters and we found a statistically significant positive correlation with WBC, ploymorphs, lymphocytes and mean platelet volume and a statistically significant negative correlation was observed with platelet count among the diabetic group whereas among the control group there were no statistical significant correlation between HbA1c and the other hemotological parameters.