Abstract

Objective To study the level of urinary 8-hydroxy deoxyguanosine (8-OHdG) in type 2 diabetic patients with or without coronary heart disease.Methods 150 cases were selected from hospitalized patients and the physical examination center in the General Hospital of Tianjin Medical University and were divided into diabetic group (n =50,male 25,female 25),diabetic patients with coronary heart disease group (n =50,male 25,female 25) and healthy control group (n =50,male 25,female 25).24 hours urine were collected,then urinary 8-OHdG was tested with enzyme linked immunosorbent assay,and urine creatinine was tested with trinitrophenol method.Blood glucose,creatinine,uric acid and blood urea nitrogen with enzymatic method,and HbA1 c with HPLC method,and fibrinogen (FIB) with Clauss method were also tested.Results Compared with normal control group [(6.97 ± 2.13) ng/mgCr],urinary 8-OHdG was increased in diabetes group [(12.26 ± 3.57) ng/mgCr] and diabetic coronary heart disease group [(14.41 ± 2.84) ng/mgCr].While compared with diabetes group,urinary 8-OHdG was significantly elevated in diabetic coronary heart disease group.Compared with healthy control group,the level of systolic blood pressure,body mass index,HbAlc,fasting blood glucose,2 hour postprandial blood glucose,triglyceride and fibrinogen were significantly higher in diabetic group and diabetic coronary heart disease group (all P ＜ 0.01),and the level of high density lipoprotein-cholesterol was lower (P ＜ 0.05).Compared with diabetes group,the level of HbA1c,2 h postprandial blood glucose and fibrinogen were significantly higher in diabetic coronary heart disease group (all P ＜ 0.01).Urinary 8-OHdG was positively related to systolic blood pressure,waist circumference,body mass index,triglyceride,fibrinogen,HbA1 c,fasting plasma glucose and 2 h postprandial blood glucose.After stepwise regressive analysis,HbA1c had significant effect on the levels of urinary 8-OHdG (P ＜ 0.001).Conclusions The level of urinary 8-OHdG is increased in patients with type 2 diabetes mellitus and diabetic patients with coronary heart disease.Urinary 8-OHdG might be a new marker for evaluating diabetic atherosclerosis. Key words: 8-Hydroxy-2'-deoxyguanosine ; Diabetes mellitus ; Coronary heart disease ; Atherosclerosis ; Oxidative stress

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