Abstract
Objective To explore the relationship between transmembrane 7 superfamily member 4 (TM7SF4) gene and breast cancer signal pathway of proliferation, invasion and metastasis. Methods After downexpressed TM7SF4 gene in michigan cancer foundation-7 (MCF-7) breast cell, screening high correlation functional gene and signal pathway by Real-time quantitative polymerase chain reaction array (qPCR array). Results After down-expressed TM7SF4 gene, there were 17 differentially expressed genes in screened 91 genes, TM7SF4 was closely related to 5 genes in the 17 kinds of differentially expressed genes using gene enrichment analysis. TM7SF4 geng was also related to tripartite motif protein family 24 (TRIM24) and tumor suppressor protein (TP53). In TRIM24 gene sets, CD44 variant 6 (CD44v6), cyclin dependent kinase inhibitor 1A (CDKN1A), Caspase-1 (CASP1) and insulin like growth factor 2 (IGF2) were obviously increased by TRIM24 signal pathway (P=0.006, 0.001, 0.002, 0.001); In TP53 gene sets, B-cell lymphoma-2 (bcl-2)-associated X (bax), CDKN1A and CASP1 were significantly increased by TP53 signal pathway (P=0.004, 0.001, 0.002). Conclusion TM7SF4 gene develops its role by TRIM24 and TP53 signal pathways in breast cancer cell line. Key words: Real-time quantitative polymerase chain reaction array; Breast cancer; Signal pathway
Published Version
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