Abstract
Objective To explore whether transfection of human apolipoprotein M (ApoM) gene can improve insulin sensitivity in the Goto-Kakizaki (GK) rats.Methods Hunan ApoM gene was inserted into lentivirus to construct LV4 (GFP)-ApoM expression vector,which was transfected into the 293T cells for 24 h.Polymerase chain reaction(PCR) was applied for analyzing the expression of genes those related to the type 2 diabetic mellitus.Ten rats were divided randomly into two groups [LV4 (GFP)-NC group and LV4 (GFP)-ApoM group] (n =5 each).GK rats were injected with the lentiviral vector-mediated 5 × 108 TU human ApoM overexpression system,and insulin tolerance test (ITT) was assessed.Human ApoM expression was detected by real-time quantitative polymerase chain reaction (Real-time PCR).Results The gene related to insulin resistance,mitogen-activated protein kinase 8 (MAPK8) was down-regulated to 2.11-fold (P ＜ 0.05),and insulin like growth factor 2 (IGF2) down-regulated to 1.23-fold (P ＜ 0.05),while ApoM expression levels in 293T cells transfected with human ApoM gene were increased to 79.43-fold (P ＜ 0.01).Human ApoM mRNA was significantly expressed in the lung of GK rats in LV4 (GFP)-ApoM group.Interestingly,ITT analysis showed that the time of blood glucose dropped to 50％ from baseline value in GK rats transfected with human ApoM gene (60 min) was shorter than that of control rats (90 min).Conclusion Overexpression of ApoM gene could improve insulin sensitivity of individuals with type 2 diabetes. Key words: Apolipoprotein M; Type 2 diabetes; Insulin sensitivity
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call