Abstract
Low molecular proteins contained in the urine of the patients of multiple myeloma and diabetes mellitus were studied physicochemically and immunologically. Urinary low molecular proteins, approximately 10, 000 to 50, 000 in molecular weight, were concentrated about 100 times by ultrafiltration.Immunoelectrophoresis of these low molecular proteins revealed fractions common in antigenic properties with albumin, α1-antitrypsin, ceruloplasmin, transferrin, IgG and Bence-Jones protein. The low molecular weight albumin was separated and purified by chromatography on Sephadex G-75 and DEAE-cellulose. Purity of this albumin was checked by double immunodiffusion and polyacrylamide gel disc electrophoresis. The approximate molecular weight of this albumin was measured as 10, 000 by ultracentrifugal and light scattering analysis. The ability of the low molecular fraction to induce formation of antibody was confirmed with experimental inoculation to rabbits.
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