Abstract
BackgroundTo identify low molecular weight urinary proteins capable of detecting diabetic nephropathy patients which may predict renal alterations at early stages and prevent it from worsening further.MethodThree hundred ninety (390) age-matched subjects were divided into 8 groups depending upon duration of diabetes and the severity of renal damage. Urinary proteome profile of all subjects was determined with the help of microfluidic array. Participants with similar profile were further selected to study proteome map of urinary low molecular weight proteins with the help of 2 dimensional gel electrophoresis.ResultsOut of 390 total patients 268 patients showed a similar one dimensional proteomic pattern. Further, two-dimensional urinary proteomic pattern of these patients with molecular weight < 50 kDa was studied. Eight proteins with molecular weight 11, 15, 17, 23, 34, 38 and 46 kDa were identified with MALDI-QTOF. These low molecular weight proteins showed gradual increase in urinary excretion along with the duration of diabetes and severity of renal damage.ConclusionThe study concludes that proteomic analysis might be a useful tool for detecting some novel markers capable of detecting patients susceptible to diabetic nephropathy in the early phase.
Highlights
Type 2 diabetes mellitus (T2DM) patients are frequently diagnosed with well established microalbuminuric stage due to its inadequate diagnosis and prognosis [1]
The study concludes that proteomic analysis might be a useful tool for detecting some novel markers capable of detecting patients susceptible to diabetic nephropathy in the early phase
Microalbumin, serum creatinine, and estimated GFR (eGFR) were within normal range in T2DM patients with 0–5, 5–10, 10–15 and 15–20 years of diabetes duration
Summary
Type 2 diabetes mellitus (T2DM) patients are frequently diagnosed with well established microalbuminuric stage due to its inadequate diagnosis and prognosis [1]. Urinary proteomics has recently been applied to understand pathophysiology and complexity of pathogenic mechanisms during T2DM induced nephropathy [3, 4]. The proteomic techniques are considered sensitive capable of detecting low abundance proteins. We compared two-dimensional urinary proteomic pattern of proteins with molecular weight < 50 kDa. For detecting the earliest possible LMWP, T2DM patients without any secondary complications (T2DM duration 1–20 years), and T2DM patients with microalbuminuria and nephropathy as a secondary complication were included. To identify low molecular weight urinary proteins capable of detecting diabetic nephropathy patients which may predict renal alterations at early stages and prevent it from worsening further
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