Abstract
The metabolism of hepatic mRNA was investigated in order to determine whether there are differences in the turnover of mRNA associated with the two populations of polyribosomes, free and membrane-bound polyribosomes, of liver. The incorporation of [6- 14C]orotic acid into hepatic mRNA was studied by two methods: (1) selective labeling of mRNA after using a low dose of actinomycin D, and (2) selective digestion of mRNA by ribonuclease using isolated polyribosomes from livers of rats with or without treatment of actinomycin D. These studies indicated that the hepatic mRNA associated with free polyribosomes became more highly labeled than mRNA of membrane-bound polyribosomes during the 0.5–3-h time course of labeling with [6- 14C]orotic acid. The present results along with previous findings of selective disaggregation of free but not of membrane-bound polyribosomes after actinomycin D treatment indicate that the average half-life of mRNA associated with free polyribosomes appears to be relatively short-lived compared to mRNA associated with membrane-bound polyribosomes.
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