Studies on the effects of nuclear background and tissue specificity on RNA editing of the mitochondrial ATP synthase subunits α, 6 and 9 in fertile and cytoplasmic male-sterile (CMS) wheat

Abstract

RNA editing occurs in transcripts encoded by the mitochondrial genome in all major groups of land plants and is characterised by C-to-U editing. When editing occurs, the amino acid is usually altered because the nucleotide modification is generally in the first or second position and the amino acid change improves the conservation of the predicted protein as compared with other organisms. In order to assess the effect of the nuclear background and tissue specificity of the RNA editing, we have analysed the RNA editing of the transcripts encoding for the mitochondrial ATPsynthase subunits atpα, atp6, and atp9 in euplasmic and alloplasmic cytoplasmic male-sterile (CMS) wheat lines. The editing of atpα was detected in 6 codons. Although partially edited transcripts were found in all the cytoplasms, a larger number of partially edited clones were present in the CMS cytoplasm. The RNA editing of atp6 occurred in 12 codons, and whereas in the euplasmic Triticum timopheevi all the clones were fully edited, in the CMS line 17% of the clones were only partially edited, suggesting an effect of the nuclear background on RNA editing efficiency. In atp9 transcripts, 8 codons were modified by RNA editing. When the RNA editing of the atp9 transcripts extracted from various tissues was compared, only fully edited clones were detected in embryos, roots, shoots, and anthers in the euplasmic wheat lines, whereas in the transcripts obtained from the CMS line, 19% of the clones were partially edited. This study reveals that RNA editing efficiency can be affected by the tissue and nuclear background. The mechanism by which incomplete and complete edited transcripts accumulate may indicate a link between the transcription kinetics and RNA editing which can be affected by nuclear background and tissue specificity.