Studies on the Antioxidant and Cytotoxic Potentials of the Peel Extract of Dacryodes rostrata

Rahma Micho Widyanto,Lulu Safira,Cleonara Yanuar Dini,Syifa Aulia Mardhiyati,Nur Faadiyah Sofian,Primaridiana Pradiptasari,Wahyu Diah Proborini,Gunadi Gunadi,A.A.C Pramana,F Hafidz As Shidieq,Afiahayati Afiahayati,R Dharmastiti,T.R Nuringtyas,A Gusnanto,J Yunus,P Indrasetiawan,W.A Kusuma,Y Ophinni,T Yamada

doi:10.1051/bioconf/20214107005

Abstract

Breast cancer is one type of cancer that causes the highest death in women in Indonesia. Alternative herbal-based cancer treatments have been developed, one of which is using fruits. Dacryodes rostrata, a fruit commonly consumed by residents in Kalimantan, is rich in antioxidants such as flavonoids and phenolics. The purpose of this study was to determine the value of antioxidant and cytotoxic activity of water and ethanol extract of D. rostrata peel against T-47D breast cancer cell lines. The fruit extraction was carried out by using maceration method. Antioxidant activity test using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and cytotoxic test using MTT method (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenitetrazolium bromide) assay. The results showed that D. rostrata water and ethanol extract had antioxidant activity with IC50values 121.7 ppm and 59.27 ppm, respectively. While cytotoxic effect on T-47D cells with IC50 values of 322.55 ppm and 143.02 ppm, respectively. This study showed that D. rostrata peel water extract had moderate antioxidant activity and moderate cytotoxic effect against T-47D breast cancer cells in vitro which could be used as a chemo preventive to prevent and inhibit cancer cell growth.

Highlights

Breast cancer is a condition of the emergence of malignant tumours originating from the breast glands
Breast cancer is one type of cancer that causes the highest death in women in Indonesia
Breast cancer in Indonesia was ranked first in the incidence of new cases and ranked second in deaths from cancer where the prevalence was highest among females (30.9%) [1]

Summary

Introduction

Breast cancer is a condition of the emergence of malignant tumours originating from the breast glands. Cancer treatment efforts can be done through radiation, surgery, chemotherapy, and hormone therapy. The therapy that is considered the most effective for breast cancer is chemotherapy This therapy has side effects that do kill cancer cells and kill normal cells in the body, causing nausea and vomiting, and trigger cachexia [2]. To overcome these problems, cancer treatment can be pursued with herbal treatment, a treatment carried out by utilizing materials from plants [3]

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