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Abstract
Acacia auriculiformis is a plant which often found in Indonesia. Acacia leaves have been known to have high antioxidant content. The phenolic compound of A. auriculiformis is the largest of these plants and phenolic compound have potential as anticancer protective agents. The aims of this study were to determine the phenolic compound, antioxidant activity, cytotoxic and antiproliferative activity of acacia leaves extract against T47D and MCF-7 breast cancer cell lines. Extraction was carried out by maceration method using methanol as a solvent. Phenolic compounds were examined by Thin Layer Chromatography (TLC) and antioxidant activity was measured using DPPH assay, followed by cytotoxicity and antiproliferation test measured by MTT Assay to obtain the IC50 value and percent of antiproliferation. Based on TLC, A. auriculiformis methanol leaves extract contain a phenolic compound with Rf value of 0.85 compared to gallic acid. ÃÂ Antioxidant activity A. auriculiformis with IC50 value of 9 ppm is classified as high antioxidant activity and MTT assay results for cytotoxicity with IC50 value of 273.8 and 31.26 ppm for T47D and MCF-7 cells, respectively. Antiproliferative analysis also showed high values for MCF-7 cells. These data have shown that methanol leaves extract of A. auriculiformis has high antioxidant activity and inhibited MCF-7 cell proliferation but not T47D breast cancer cell lines.ÃÂ
Highlights
Acacia auriculiformis or acacia plants are included in the Fabaceae family and commonly found in Indonesia
Based on the research conducted by Chew et al (2011) regarding antioxidant activity, it is known that methanol leaves extract of A.auriculiformis has an IC50 value of 161 ±30 μg/mL and Sravanthi et al (2014) shown cytotoxic activity (Brine Shrimp Lethality Bio-assay) with IC50 value of 0.55 g/mL for the leaves methanol fraction of A
T47D breast cancer cells respond to phenolic compounds by interaction with Aryl hydrocarbon receptor (AhR), inhibition of nitric oxide synthase (NOS) and induction of cell apoptosis through the Fas/FasL pathway (Kampa et al, 2003)
Summary
Acacia auriculiformis or acacia plants are included in the Fabaceae family and commonly found in Indonesia. T47D breast cancer cells respond to phenolic compounds by interaction with Aryl hydrocarbon receptor (AhR), inhibition of nitric oxide synthase (NOS) and induction of cell apoptosis through the Fas/FasL pathway (Kampa et al, 2003). Both cells have the ability to express estrogen receptors (ER). This research was conducted to study the antiproliferative effect of Acacia auriculiformis extract on breast cancer cell lines. Antiproliferation Test Antiproliferation were measured by MTT Assay, in the same condition with cytotoxic test with concentrations 400, 200, 100, 50, and 25 ppm using DMSO as a solvent. While antiproliferation test was expressed by percent of inhibition proliferation inhibition of T47D and MCF-7 cells
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