Abstract
1. Methylated bases in ribosomal RNA (rRNA) were estimated by a method consisting of (a) in vivo methyl labelling of the rRNA, (b) isolation of the rRNA, (c) acid hydrolysis of the RNA in the presence of carriers, (d) separation of the hydrolysis mixture by two-dimensional chromatography and (e) estimation of the radioactivity in the separated spots. Ribose-methylated nucleotides were estimated separately. 2. The procedure was used to compare five strains of Escherichia coli and two strains of Saccharomyces cerevisiae. The two organisms differ markedly in the distribution of methylated compounds in their rRNA, while strains of the same organism are very similar. The content of O- ribose methyl groups was high in the yeast strains but rather low in the bacteria.
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