Abstract
Preparations from cotyledons of unimbibed peanut seed show a low level of amino acid incorporation into protein. Similar preparations from imbibed seed exhibit increased activity. RNA extracted from germinating peanut cotyledons and then eluted after ribosomal RNA on the methylated albumin-kieselguhr (MAK) column had the highest template activity. This fraction stimulates amino acid incorporation into protein in an in vitro system. Isolated messenger RNA binds to monoribosomes obtained from dry seed and the polyribosomes thus produced are active in protein synthesis. It is suggested from these results that native mRNA from peanut cotyledons forms a stable complex with monoribosomes in the presence of Mg 2+. The reaction does not require an ATP energy source or 105 000 × g supernatant enzymes.
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