Studies on acellular bovine glomeruli: Isolation, chemical composition, and demonstration of collagen with an unusual hydroxylysine: Hydroxyproline ratio

Abstract

Bovine glomeruli from which most cellular elements were removed contained a protein that shared several properties with collagen. These included the presence of hydroxylysine and hydroxyproline, digestion with collagenase, dissolution by heating, resistance to tryptic digestion, and an electron diffraction pattern characteristic of collagens. A distinctive feature of glomerular collagen was an unusually high content of hydroxylysine expressed as the hydroxylysine: hydroxyproline ratio. This collagen constituted about one half of the acellular glomerular mass.