The Chemical and Immunologic Properties of a New Collagen and a Glycoprotein in Glomerular Basement Membrane.

Abstract

s1 May 1966The Chemical and Immunologic Properties of a New Collagen and a Glycoprotein in Glomerular Basement Membrane.Nicholas A. Kefalides, M.D., Ph.D.Nicholas A. Kefalides, M.D., Ph.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-64-5-1166_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe amino acid composition of the isolated, normal canine glomerular basement membrane resembled that of tendon collagen but differed in being lower in glycine, proline, and hydroxyproline but higher in hydroxylysine. The hydroxylysine: hydroxyproline ratio in basement membrane was 0.42 compared with 0.07 in collagen. Carbohydrate makes up approximately 10% of basement membrane by weight, while collagen contains 0.6% carbohydrate. Basement membrane was solubilized by reduction of disulfide bonds in 8 M urea followed by alkylation. The amino acid and carbohydrate composition of the soluble fraction was similar to that of intact basement membrane. The presence of two protein components,... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Chicago, Illinois PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 May 1966Volume 64, Issue 5Page: 1166-1166KeywordsAmino acidsBasement membraneCarbohydratesGlycineGlycoproteinsProteinsTendonsUrea Issue Published: 1 May 1966 PDF downloadLoading ...