Abstract
In a recent study, Kefalides and Winzler (1964, 1965) reported the presence of a glycoprotein associated with a collagen-like protein in glomerular basement membrane of dogs. The basement membrane was solubilized by extraction with 8 M urea or by reduction and alkylation of disulfide bonds in 8 M urea. Amino acid analysis of basement membrane and its soluble fraction revealed the presence of a high hydroxylysine:hydroxyproline ratio (0.42 in basement membrane compared with 0.07 in mammalian collagen). Intact basement membrane contained 5.52% hexose, 1.4% glycosamine, 2.1% sialic acid, and 0.75% fucose. The amino acid and carbohydrate composition of the soluble fractions did not differ significantly from that of intact basement membrane. Dische et al . (1965) reported the presence of glucose and galactose linked to collagen and of a sialofucohexosaminoglycan in human glomerular basement membrane. The present report deals with the separation of two components from canine glomerular basement membrane. One is a glycoprotein and the other a collagen with a much higher hydroxyproline and hydroxylysine content than is found in other mammalian collagens.
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