Abstract
This chapter presents an investigation of monocyte Fc receptor avidity and monocyte-mediated antibody-dependent cellular cytotoxicity (MMADCC) in man. Peripheral blood monocytes from normal subjects and patients with various immunodeficiency diseases, including the Wiskott–Aldrich syndrome (WAS) and intestinal lymphangiectasia (IL), were studied in an analysis presented in the chapter. In addition, pleural fluid macrophages were studied in one patient with IL. Mononuclear cells were incubated with 51 Cr-labeled human blood group B erythrocytes in the presence of human anti-blood group B antiserum and the number of targets killed determined from the specific release of 5l Cr into the medium. In this system, monocytes but not lymphocytes act as effector cells and the amount of killing observed correlates with the number of monocytes in the mononuclear cell effector population. The results showed that MMADCC requires the Fc portion of the antibody. However, as seen in the WAS patients, binding of the antibody-coated target to the monocyte is not in itself sufficient for lysis to occur, indicating that while antibody dependent cellular cytotoxicity requires an Fc receptor, the mere presence of a functional Fc receptor is not enough to trigger the cytotoxic event.
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