Abstract
An antibody raised against two subunits ( M r, 48000 and 25000) of NADH dehydrogenase from Paracoccus denitrificans cross-reacts with one of more than 20 polypeptides that form the bovine heart mitochondrial NADH dehydrogenase. The cross-reacting subunit has M r, 51000 and is believed to be the NADH-binding subunit of the enzyme. Antibodies raised against certain subunits of the bovine heart NADH dehydrogenase were tested for cross-reactivity with P. denitrificans cytoplasmic membranes. Of those tested, only one, an antibody specific for the 49 kDa subunit of mitochondrial NADH dehydrogenase, cross-reacted with the bacterial membranes. It recognised a polypeptide of approximate M r, 46000. This is an indication for a previously undetected third subunit of NADH dehydrogenase from P. denitrificans. The immunological crossreactions indicate that the NADH dehydrogenases from P. denitrificans and bovine heart mitochondria are related structurally.
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