Abstract
The separation and structural analysis of 21 permethylated trisaccharides by gas-liquid chromatography (g.l.c.) and combined gas-liquid chromatography-mass spectrometry (g.l.c.-m.s.) is described. The compounds include examples of straight-chain trisaccharides having all the 9 possible combinations of (1→6)-, (1→4)-, and (1→3)-linkages between the monosaccharide units. Analysis of a few miscellaneous compounds having (1→2)-linkages, containing pentoses or having a branched-chain structure, is also presented. The results show that g.l.c. (stationary phases SE-30 or OV-22) is well suited for resolving trisaccharide mixtures into their components and for their preliminary identification. Analysis by g.l.c.-m.s. discloses the molecular weights of the monosaccharide units, the sequence of monosaccharides if they have different masses, and the presence of chain branching. The position of the glycosidic linkage next to the reducing end of straight-chain trisaccharides can generally be established by m.s., whereas differentiation of (1→6)- and (1→4)-linkages next to the non-reducing end may require previous knowledge of the stereochemistry of the sugar units. The use of g.l.c.-m.s. in studies of partial hydrolysates of polysaccharides is discussed.
Published Version
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