STRESS-PROTECTIVE ACTIVITY OF THE COMPLEX REMEDY IN ACUTE IMMOBILIZATION STRESS

Abstract

Relevance. The range of medicinal remedies in the traditional Tibetan medicine contains several tens of formulae named bchud len. They are multicomponent remedies of natural origin having a stimulating non-specific effect on the human body. The analysis of such formula under the name ‘Rejuvenating-35’ has been presented in the paper. The complex remedy on the base of this formula was developed. Its composition includes 14 species of the plants and chitosan as a source of mineral substances. Previous phytochemical studies have revealed that the given remedy contains a wide spectrum of biologically active substances, namely triterpene saponins, flavonoids, ether oils, tannins, polysaccharides, etc. The aim of the present work was to evaluate stress-protective activity of the complex remedy in acute restraint stress. Material and methods. Experiments were carried out on white Wistar rats. The acute stress was simulated by immobilization of animals in supine position for 18 hours. The tested remedy was introduced in the form of decoction for 7 days before exposure to stress. Results. It has been established that the course administration of the complex remedy decreases the manifestation of the Selye’s triad signs: 26% decrease of adrenal glands hypertrophy, 45% and 60% decrease in involution of thymus and spleen respectively as compared to the data in the control group of animals. Besides, its administration prevents the development of rough destructions in the stomach mucosa in the form of erosions and linear ulcers. Against the background of the complex remedy administration there was noted twofold decrease of TBA-active products concentra-tion, fourfold increase of superoxide dismutase activity, 30% increase of catalase activity and the content of the reduced glutathione was 30% in-creased as compared to the data in the control. Conclusions. The preventive administration of the complex remedy has stress-protective effect against the background of acute restraint stress. Molecular-cell mechanisms of its anti-stress effect are connected with the inhibition of free-radical oxidation processes and the increased activity of the endogenic antioxidant system.